Directions for Laboratory Work in Physiological Chemistry

ps of potassium- mercuric-iodide. (e) To successive portions of 5 c.c. of the albumin solution add a few drops of CuSO4; neutral and basic lead acetate; HgCl2; trichloracetic acid. Make careful notes of the results of the above reactions, and where possible write equations. SIMPLE PROTEIDS. Albumins. (?) Coagulation. Heat 5 c.c. of the albumin solution and then add one or two drops of acetic acid. The albumin separates out in an insoluble, flocky (coagulated) form. Why is the addition of the acetic acid necessary? Try to dissolve the coagulum in some of the ordinary proteid solvents. Make 5 c.c. of the albumin solution faintly alkaline, and heat. Note differences. (?) To 10 c.c. of the albumin solution add (NH4)2SO4 to saturation. Note result and compare with experiment (c) 5, p. 11. Filter off the precipitate and test the filtrate with the .Biuret test. (c) To 10 c.c. of the albumin solution add MgSO4 to saturation. Compare this result with that of experiment (c) 4,p. 11. Now add 2 drops of acetic acid. What is the precipitate? (d) Test the albumin solution for loosely combined sulphur. (e) Warm some dried albumin in a dry test-tube to about 100 C. Then try to dissolve it in water. Why does not the albumin coagulate by the heat? Globulins. (a) Try two proteid color reactions and three precipitation reactions; see if the solution will coagulate. (b) Globulins are insoluble in water but dissolve in weak salt soluti...